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A comprehensive and intensive course in light
microscopy for researchers in biology, medicine, and material sciences.
This course provides a systematic and in-depth examination of the
theory of image formation and application of video and digital methods
for exploring subtle interactions between light and the specimen. This
course emphasizes the quantitative issues that are critical to the
proper interpretation of images obtained with modern wide-field and
confocal microscopes. This course is limited to 32 students.
Laboratory exercises, demonstrations, and discussions include: (1)
geometrical and physical optics of microscope image formation including
Abbe’s theory of the microscope and Fourier optics; (2) interaction of
light and matter; (3) phase contrast polarization and interference
microscopy for the nondestructive analysis of molecular and
fine-structural organization in living cells; (4) fluorescence
microscopy, quantification of fluorescence, and GFP; (5) principles and
application of digital video imaging, recording, analysis, and display;
(6) digital image processing and quantitative digital image
deconvolution; (7) ratiometric measurement of intracellular ion
concentrations; (8) confocal microscopy; and (9) new advances in light
microscopy such as FRET, FLIM, TIRF, and patterned illumination.
The program is designed primarily for: (1) university faculty,
professional researchers, postdoctoral fellows, and advanced graduate
students in the life sciences who wish to expand their experience in
microscopy and to understand the quantitative issues associated with
analysis of data obtained with optical microscopes; (2) individuals
well-grounded in the physical sciences, who wish to exploit microscopy
techniques for analyzing dynamic fine-structural and chemical changes;
and (3) industrial scientists and engineers interested in advancing the
design of equipment and techniques involving video and digital
microscopy.
Lectures are followed by small group laboratory sessions and
demonstrations. As a result, students will have opportunities for
extensive hands-on experience with state-of-the-art optical,
electronic, and digital imaging equipment guided by an experienced
staff from universities and industry.
Partial support is provided by
Howard
Hughes Medical Institute
2012 Course Faculty & Lecturers:
Michael Davidson, Florida State University
Graham Dempsey, Harvard University
Chris Dunsby, Imperial College London
Kevin Eliceiri, University of Wisconsin
Shinya Inoué, Marine Biological Laboratory
Khuloud Jaqaman, Harvard Medical School
John Murray, University of Pennsylvania
Rudolf Oldenbourg, Marine Biological Laboratory
Rudolf Rottenfusser, Carl Zeiss MicroImaging, LLC
Curtis Rueden, University of Wisconsin
Edward (Ted) Salmon, University of North Carolina at Chapel Hill
Wendy Salmon, Whitehead Institute for Biomedical Research
Greenfield Sluder, UMass Medical School
Jason Swedlow, University of Dundee
Jenny Tam, Massachusetts General Hospital
Justin Taraska, National Institutes of Health
Elizabeth Williams, Rockefeller University
David Wolf, Radiation Monitoring Devices
TAs:
Sebastien Besson, Harvard Medical School
Stephen Douthwright, UMass Medical School
Anna Krzywicka-Racka, UMass Medical School Worcester
Cassandra Rogers, Children's Hospital Boston
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