This course is designed primarily for research scientists, postdoctoral trainees, and advanced graduate students in animal, plant, medical, and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and digital imaging will benefit greatly from the course. Some prior theoretical or practical understanding of the basic principles of optics and microscopy is necessary. This 10 day course is limited to 26 students. It consists of correlated lectures, laboratory exercises, demonstrations, and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce high quality digital video, and digital records for documentation and analysis.
Topics to be covered include: (a) fundamental principles of microscope design, image formation, resolution, contrast; (b) bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy; (c) cameras, signal to noise ratio, digital image recording, processing and analysis, multispectral imaging; (d) advanced fluorescence-- fluorescent probes, TIRF, FRET, FLIM, FRAP, polarization of fluorescence, fluorescence correlation spectroscopy; (e) digital image restoration/deconvolution, and 3-D imaging principles, confocal scanning microscopy, multiphoton excitation fluorescence microscopy; application of the optical methods to live cells will be emphasized. Other specimens also will be covered.
Students will have direct hands-on experience with state-of-the-art microscopes, digital cameras, recorders, and image processing equipment provided by major optical, electronics, and software companies. Instruction will be provided by experienced staff from universities and industry. Students are encouraged to bring their own biological and material specimens, and to discuss individual research problems with the faculty.
2012 Academic Faculty and Assistants
Gary Borisy, Director and President, Marine Biology Laboratory
Richard, Day, Indian University School of Medicine
Joseph DePasquale, Morphogenyx
Paul Goodwin, Applied Precision Instruments
Fred Lanni, Carnegie Melon University
Jennifer Lippincott-Schwartz, National Institutes of Health
James McNally, National Institutes of Health
Butch Moomaw, Hammamatsu Corp.
John Murray, Indiana University
HariShroff, National Institutes of Health
Wade Sigurdson, University at Buffalo
Clare Waterman, National Institutes of Health
Lindsay Case, Naitional Institutes of Health
Julia Hum, Indiana University School of Medicine
Colleen Skau, National Institutes of Health