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An intensive and comprehensive
laboratory-oriented course in cellular and molecular neurobiology
intended for predoctoral students, postdoctoral or clinical
researchers, and young investigators beginning independent research
careers. Limited to 14 students.
A hallmark of this course is the extensive lab work done in close
collaboration with expert faculty. The course is divided into three
sections: Electrophysiology, Imaging, and Molecular Neurobiology. These
are taught by separate groups of faculty, usually six in each section,
and with many guest lecturers. Each section begins with specific
training in core laboratory techniques; students then undertake one- to
two-week directed or independent projects using the methods they have
learned. Didactic lectures are combined with laboratory experience in
order to establish a strong conceptual foundation for each section. A
typical day has 3 hours of lecture and 10 hours of lab.
Electrophysiological methods focus on patch-clamp and sharp electrode
recordings, performed on neurons in a variety of preparations,
including tissue culture, brain slices, isolated squid synapses, rat
cochlea, or whole fish. Optical methods include calcium imaging,
confocal and 2-photon microscopy, videomicroscopy, and electron
microscopy. Molecular techniques emphasize the use of forward and
reverse genetics in diverse systems such as Drosophila, C. elegans,
zebrafish,
chick
embryos,
and primary cells in culture. The impact of
genetic manipulations are assayed by real time PCR, laser
microdissection, single cell PCR, in situ hybridization, and a
variety of immunotechniques in addition to incorporating
electrophysiological and imaging techniques.
The goal of the course is to emphasize the strengths of a
multidisciplinary approach for studying the function of the nervous
system at the cellular and molecular levels.
This course is supported with funds provided by
National
Institute
of
Neurological
Disorders and Stroke, NIH
Howard
Hughes Medical Institute
The Grass Foundation
International Brain Research Organization
2012 Faculty:
Aizenman, Carlos, Brown University
Araneda, Ricardo, University of Maryland
Bautista, Diana, University of California, Berkeley
Buchanan, JoAnn, Stanford University
Commons, Kathryn, Children's Hospital/Harvard Medical School
Diaz, Elva, University of California, Davis
Eaton, Benjamin, University of Texas Health Science Center, San Antonio
Edwards, Tyson, Yale University
Galbraith, Catherine, National Institutes of Health
Galbraith, James, National Institutes of Health
Hammarlund, Marc, Yale University
Hoppa, Michael, Weill Cornell Medical College
Khodakhah, Kamran, Albert Einstein College of Medicine
Kim, Charlie, University of California, San Francisco
Lichtman, Jeff, Harvard University
Llano, Isabel, CNRS
Marty, Alain, CNRS
Micheva, Kristina, Stanford University
Misgeld, Thomas, Technical University Munich
Oertner, Thomas, Center for Molecular Neurobiology
Parrish, Jay, University of Washington
Peterka, Darcy, Columbia University
Pratt, Kara, University of Wyoming
Sack, Jon, University of California, Davis
Sagasti, Alvaro, University of California, Los Angeles
Smith, Stephen, Standford University School of Medicine
Stuurman, Nico, University of California, San Francisco
Von Zastrow, Mark, University of California, San Francisco
Yasuda, Ryohei, Duke University Medical Center
Zuo, Yi, University of California, Santa Cruz
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