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architectural dynamics

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Laboratory of Rudolf Oldenbourg

Phone (508) 289-7426
Fax (508) 540-6902
E-Mail rudolfo@mbl.edu
Address Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
Research Interests:
Our research is inspired by advanced optical methods to study the architectural dynamics in living cells. We are developing a new type of polarized light microscope, the LC-PolScope, for the analysis of molecular order directly in living cells with unprecedented sensitivity, resolution, and speed. Based on polarization measurements, we gain insight into submicroscopic structural parameters and non-invasively create contrast where structures are otherwise invisible. We seek interdisciplinary collaborations to conduct research in physical optics for the interpretation of image content and in computational methods for image enhancement and restoration. These physical and engineering projects are stimulated and guided by biological inquiries into the structural basis of cell function.

Senior Scientist Rudolf Oldenbourg, Cellular Dynamics Program

Rudolf Oldenbourg Education:

1981   Ph.D. Physics, University of Konstanz, Konstanz, Germany
   
1976   Diplomphysiker, Technical University, Munich, Germany


Selected Publications:

1. LaFountain, J. R., Jr., C. S. Cohan, and R. Oldenbourg (2011) Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy, Molecular Biology of the Cell. In print

2. DeMay, B. S., N. Noda, A. S. Gladfelter and R. Oldenbourg. 2011. Rapid and quantitative imaging of excitation polarized fluorescence reveals ordered septin dynamics in live yeast. Biophys. J. 101: 985-94.

3. DeMay, B. S., X. Bai, L. Howard, P. Occhipinti, R. A. Meseroll, E. T. Spiliotis, R. Oldenbourg and A. S. Gladfelter. 2011. Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals. J. Cell Biol. 193: 1065-81.

4. Valm, A. M., J. L. Welch, C. W. Rieken, Y. Hasegawa, M. L. Sogin, R. Oldenbourg, F. E. Dewhirst and G. G. Borisy. 2011. Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging. Proc Natl Acad Sci U S A 108: 4152-7.

5. Miyata, H., N. Noda, D. J. Fairbairn, R. Oldenbourg and R. A. Cardullo. 2010. Assembly of the fluorescent acrosomal matrix and its fate in fertilization in the water strider, Aquarius remigis. J. Cell. Physiol.

6. Henson, J. H., D. Cheung, C. A. Fried, C. B. Shuster, M. K. McClellan, M. K. Voss, J. T. Sheridan and R. Oldenbourg. 2009. Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network. Cell Motil. Cytoskeleton 66: 679-92.

7. Barry, E., Z. Dogic, R. B. Meyer, R. A. Pelcovits and R. Oldenbourg. 2009. Direct Measurement of the Twist Penetration Length in a Single Smectic A Layer of Colloidal Virus Particles. J Phys Chem B 113: 3910-3.

8. Guo, Y., Y. Liu, R. Oldenbourg, J. X. Tang and J. M. Valles, Jr. 2008. Effects of osmotic force and torque on microtubule bundling and pattern formation. Physical Review E (Statistical, Nonlinear, and Soft Matter Physics) 78: 041910.

9. Oldenbourg, R. 2008. Polarized light field microscopy: an analytical method using a microlens array to simultaneously capture both conoscopic and orthoscopic views of birefringent objects. J. Microsc. 231: 419-32.

10. Henson, J. H., C. A. Fried, M. K. McClellan, J. Ader, J. E. Davis, R. Oldenbourg and C. R. Simerly. 2008. Bipolar, anastral spindle development in artificially activated sea urchin eggs. Dev. Dyn. 237: 1348-58.

11. Oldenbourg, R. 2007. Analysis of microtubule dynamics by polarized light. Methods Mol Med, 137, 111-123.

12. Janicke, M. A., L. Lasko, R. Oldenbourg and J. R. LaFountain, Jr. 2007. Chromosome malorientations after meiosis II arrest cause nondisjunction. Mol. Biol. Cell 18: 1645-56.

13. Barry, E., Z. Hensel, Z. Dogic, M. Shribak and R. Oldenbourg. 2006. Entropy-driven formation of a chiral liquid-crystalline phase of helical filaments. Phys. Rev. Lett.Phys. Rev. Lett. 96: Art. No. 018305.